Do you mean a single Glyphs file?
Did you test the font in Adobe Illustrator or InDesign? There are many details to get right, check your Font Info > Exports and see if you configured the Weight Class and Width Class correctly – I usually miss some of those. Also the order of the Axes in Font Info must be correct. You could post some screenshots of your Font Info/Masters/Exports setup perhaps we’ll spot something.
Also, the order of the Masters can affect things, see the example in this post: Slanted and Backslanted Variable Font Adobe Menu Sorting